High-performance liquid chromatography (HPLC) is an analytical test that is used to identify and quantify the various components of a mixture. A number of different industries rely on HPLC for research, development, and quality control.
In this article, we will provide an overview of HPLC and talk about why it is such an important and prevalent test.
What Is HPLC?
The concept of liquid chromatography, which involves dissolving a sample in a solution and passing it through a chromatographic column, is the basis for the HPLC test.
During the test, the components within a sample interact differently with the stationary phase in the column, resulting in their physical and chemical properties being separated. Relative to conventional liquid chromatography techniques, HPLC provides greater speed, sensitivity, and accuracy.
Industrial Application
HPLC is widely utilised in the healthcare industry for the analysis of drugs. It can determine the purity of medication, quantify the amount of active ingredients, and more. Pharmaceutical researchers can satisfy regulatory requirements and safeguard public health by using HPLC to guarantee the effectiveness, safety, and quality of drugs.
The technique is also used in biotechnology and biological sciences. It can help technicians characterise biomolecules, analyse amino acids, and more.
Furthermore, this analytical test is also utilised in environmental analysis to assess air quality, identify toxins in soil and water samples, and more.
HPLC is essential to safety testing as well as quality control in the food and beverage sector. It is utilised to ensure that products meet the standards set out by regulatory bodies that restrict the use of pesticides and other contaminants.
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